microrna-205 inhibits renal cells apoptosis via targeting cmtm4

objective(s):micrornas (mirnas) are small non-coding rna molecules that regulate gene expression. they have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in various forms of chronic kidney disease (ckd). in the present study, we elucidated the role of microrna 205 (mir-205) in cisplatin-induced renal cell apoptosis and explored the molecular mechanisms. materials and methods:the chronic interstitial nephropathy rat model was induced, and the mirna expression profile in the kidney cells from rats with ckd was screened. cisplatin-induced apoptosis in normal renal hk-2 cells was evaluated using flow cytometry, and regulation of mir-205 on target gene was validated using luciferase assay, western blot and real time pcr assays. results: we found that mir-205 expression was significantly decreased in the cells from kidney of ckd rat (p<0.01). our data showed that when mir-205 was overexpressed or silenced using the mimic or inhibitor, the percentages of apoptotic cells were suppressed or increased significantly (p<0.05), respectively. moreover, we have identified cmtm4 gene, which is involved in cell proliferation and apoptosis, as a novel target for mir-205. in addition, mir-205 could inhibit apoptosis by binding to the 3’utr of cmtm4 mrna and inhibiting its transcriptional activity. conclusion: this study elucidated that mir-205 plays an important role in the regulation of apoptosis in renal cells, suggesting a potential therapeutic target to hinder ckd development.